Identification of Circular RNAs using RNA Sequencing

Shobana Sekar; Philipp Geiger; Lori Cuyugan; Annalee Boyle; Geidy Serrano; Thomas G. Beach; Winnie S. Liang

doi:10.3791/59981

Journal

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Genetics

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Identification of Circular RNAs using RNA Sequencing

JoVE Journal
Genetics

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JoVE Journal Genetics

Identification of Circular RNAs using RNA Sequencing

11,775 Views

•

08:25 min

•

November 14, 2019

DOI:

10.3791/59981-v

DOI:

10.3791/59981-v

•

08:25 min

November 14, 2019

•

9 Views

Shobana Sekar^1,2, Philipp Geiger^1,2, Lori Cuyugan^1,2, Annalee Boyle^1,2, Geidy Serrano^2,3, Thomas G. Beach^2,3, Winnie S. Liang^1,2

¹Translational Genomic Research Institute, ²Arizona Alzheimer’s Consortium, ³Banner Sun Health Research Institute

Summary

Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.

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Identification of Circular RNAs using RNA Sequencing

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