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Um ensaio robusto baseado em reação em cadeia da polimerase para quantificar repetições de trinucleotídeo de citosina-guanina-guanina em X frágil retardo mental-1 gene
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Genetics
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JoVE Journal Genetics
A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene

Um ensaio robusto baseado em reação em cadeia da polimerase para quantificar repetições de trinucleotídeo de citosina-guanina-guanina em X frágil retardo mental-1 gene

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08:22 min

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September 16, 2019

DOI:

10.3791/59963-v

DOI:
10.3791/59963-v

•

08:22 min
September 16, 2019

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1Central Laboratory, Bao’an Maternity and Child Health Hospital,Jinan University, 2Department of Obstetrics and Gynaecology,The Chinese University of Hong Kong, 3Shenzhen Research Institute,The Chinese University of Hong Kong, 4Department of Genetics and Metabolism,Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, 5PerkinElmer Diagnostics, 6Department of Obstetrics and Gynecology, Bao’an Maternity and Child Health Hospital,Jinan University, 7Maternal-Fetal Medicine Institute, Bao’an Maternity and Child Health Hospital,Jinan University

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Wang, H., Zhu, X., Gui, B., Cheung, W. C., Shi, M., Yang, Z., Kwok, K. Y., Lim, R., Pietilä, S., Zhu, Y., Choy, K. W. A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene. J. Vis. Exp. (151), e59963, doi:10.3791/59963 (2019).

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