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06:10 min
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September 02, 2019
DOI:
1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science,Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences,Zhejiang University, 4The New Countryside Development Institute at Zhejiang University
Summary
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In questo articolo, presentiamo il protocollo descritto come ad alta risoluzione di fusione di analisi di fusione (HRM)-based Target Induced Local Lesions In ducedato In genomes (TILLING). Questo metodo utilizza cambiamenti di fluorescenza durante la fusione del DNA duplex ed è adatto per lo screening ad alto throughput di inserimento/eliminazione (Indel) e singola sostituzione di base (SBS).
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Cite this Article
Li, S., Yu, Y., Liu, S., Fu, H., Huang, J., Shu, Q., Tan, Y. Identifying Mutations by High Resolution Melting in a TILLING Population of Rice. J. Vis. Exp. (151), e59960, doi:10.3791/59960 (2019).
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