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자외선-C (UV-C) 손상에 응하여 1 차 마우스 안구 표면 세포/줄기 세포에 있는 산화 손상의 평가
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JoVE Journal Developmental Biology
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
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자외선-C (UV-C) 손상에 응하여 1 차 마우스 안구 표면 세포/줄기 세포에 있는 산화 손상의 평가

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12:59 min

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February 15, 2020

DOI:

10.3791/59924-v

DOI:
10.3791/59924-v

•

12:59 min
February 15, 2020

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1 Views
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1Stem Cells and Regenerative Medicine Centre,Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India

Tags

Oxidative DamagePrimary Mouse Ocular Surface CellsStem CellsUltraviolet-C (UV-C) DamageReactive Oxygen Species (ROS)DCFDAPropidium IodideHoechstLive Cell PermeantDead Cell PermeantUV CrosslinkerDose-response

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Cite this Article

Bose, B., Kapoor, S., Sen, U., Nihad AS, M., Chaudhury, D., Shenoy P, S. Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage. J. Vis. Exp. (156), e59924, doi:10.3791/59924 (2020).

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