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A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdefinitely) in Cancers
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Genetics
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JoVE Journal Genetics
A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdeff) in Cancers
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A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdefinitely) in Cancers

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5,446 Views

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10:49 min

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September 26, 2019

DOI:

10.3791/59910-v

DOI:
10.3791/59910-v

•

10:49 min
September 26, 2019

•

2 Views
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1Division of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute,Cincinnati Children’s Hospital Medical Center (CCHMC)

Summary

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Das Protokoll beschreibt ein in vitro muriner Karzinommodell der nicht-genetischen defekten Transkriptionsdehnung. Hier wird die chronische Hemmung von CDK9 verwendet, um die produktive Dehnung von RNA Pol II entlang der pro-entzündlichen Reaktionsgene zu unterdrücken, um das klinisch überversorgteTE-Phänomen, das bei etwa 20% aller Krebsarten vorhanden ist, zu imitieren und zu untersuchen.

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Modur, V., Singh, N., Muhammad, B. A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdeff) in Cancers. J. Vis. Exp. (151), e59910, doi:10.3791/59910 (2019).

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