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11:54 min
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July 13, 2019
DOI:
1Department of Nephrology,University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology,University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne,University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen,University Hospital Essen Hufelandstr
Summary
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Neste manuscrito, um protocolo é apresentado para executar a medida FACS-baseada da atividade transcricional de BK-Polyomavirus usando as pilhas HEK293T transfected com um plasmídeo bidirecional do repórter que expressa tdtomato e EGFP. Este método permite determinar quantitativamente a influência de novos compostos na transcrição viral.
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Cite this Article
Korth, J., Sertznig, H., Moyrer, S., Doevelaar, A. A. N., Westhoff, T. H., Babel, N., Witzke, O., Kribben, A., Dittmer, U., Widera, M. Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry. J. Vis. Exp. (149), e59755, doi:10.3791/59755 (2019).
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