Journal
/
Neuroscience
/
Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

DOI:
10.3791/59685-v

•

06:04 min

•

May 23, 2019

•

, , ,
1Department of Psychological and Brain Sciences,University of Delaware, 2Plant and Soil Sciences, Delaware Biotechnology Institute,University of Delaware

Chapters

  • 00:04Title
  • 00:45Single Immunohistochemical Reaction
  • 02:45Imaging and Protein Expression Analysis
  • 04:14Results: Detection and Measurement of Protein Expression in the Rodent Brain
  • 05:30Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol that uses near-infrared dyes in conjunction with immunohistochemistry and high-resolution scanning to assay proteins in brain regions.

Tags

Keywords: Near-infrared FluorescenceHigh-resolution ScanningProtein ExpressionRodent BrainImmunohistochemistryMolecular Signaling PathwaysAMPA/NMDA RatioCryostatParaformaldehydeTBSPrimary AntibodySecondary AntibodyNear-infrared Scanning

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59685?article_type=v

Simultaneous fMRI and Electrophysiology in the Rodent Brain
check_url/59685?article_type=v

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
check_url/59685?article_type=v

Using MazeSuite and Functional Near Infrared Spectroscopy to Study Learning in Spatial Navigation
check_url/59685?article_type=v

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
check_url/59685?article_type=v

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
check_url/59685?article_type=v

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
check_url/59685?article_type=v

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
check_url/59685?article_type=v

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster
check_url/59685?article_type=v

Personalized Needles for Microinjections in the Rodent Brain
check_url/59685?article_type=v

Conducting Hyperscanning Experiments with Functional Near-Infrared Spectroscopy

Read Article