Here, we present a protocol that uses near-infrared dyes in conjunction with immunohistochemistry and high-resolution scanning to assay proteins in brain regions.
Kimmelmann-Shultz, B., Mohmammadmirzaei, N., Caplan, J., Knox, D. Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain. J. Vis. Exp. (147), e59685, doi:10.3791/59685 (2019).