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אפיון של חלבונים לפי גודל כרומטוגרפיה הדרה מצמידים לפיזור אור רב זווית (SEC-דוליטל)
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Biochemistry
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JoVE Journal Biochemistry
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
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אפיון של חלבונים לפי גודל כרומטוגרפיה הדרה מצמידים לפיזור אור רב זווית (SEC-דוליטל)

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10:00 min

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June 20, 2019

DOI:

10.3791/59615-v

DOI:
10.3791/59615-v

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10:00 min
June 20, 2019

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1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

Tags

SEC-MALSSize-exclusion ChromatographyMulti-angle Light ScatteringMolecular WeightMacromoleculesOligomersComplexesGlycoproteinsMembrane ProteinsAbsolute MethodPeptidesPolymersProteinsNucleocapsidsPolysaccharidesVirus-like ParticlesVirusesPhosphate-buffered SalineFlow RateDRI Flow CellPurge ModeSystem CleanlinessRefractive Index

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Some, D., Amartely, H., Tsadok, A., Lebendiker, M. Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS). J. Vis. Exp. (148), e59615, doi:10.3791/59615 (2019).

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