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Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
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June 20, 2019
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This protocol describes the combination of size exclusion chromatography with multi-angle light scattering (SEC-MALS) for absolute characterization of proteins and complexes in solution. SEC-MALS determines the molecular weight and size of pure proteins, native oligomers, heterocomplexes and modified proteins such as glycoproteins.
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SEC-MALSSize-exclusion ChromatographyMulti-angle Light ScatteringMolecular WeightMacromoleculesOligomersComplexesGlycoproteinsMembrane ProteinsAbsolute MethodPeptidesPolymersProteinsNucleocapsidsPolysaccharidesVirus-like ParticlesVirusesPhosphate-buffered SalineFlow RateDRI Flow CellPurge ModeSystem CleanlinessRefractive Index
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Some, D., Amartely, H., Tsadok, A., Lebendiker, M. Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS). J. Vis. Exp. (148), e59615, doi:10.3791/59615 (2019).
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