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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
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October 07, 2020
DOI:
1Department of Biological Sciences, Faculty of Science and Engineering,Chuo University, 2Faculty & Graduate School of Urban Environmental Sciences,Tokyo Metropolitan University, 3Center for Ecological Research,Kyoto University, 4Department of Chemistry, Faculty of Science,Toho University, 5Graduate School of Integrated Arts and Sciences,Hiroshima University
Summary
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A series of methods to determine the potential DNRA rate based on 14NH4+/15NH4+ analyses is provided in detail. NH4+ is converted into N2O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.
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Kuroiwa, M., Fukushima, K., Hashimoto, K., Senga, Y., Sato, T., Katsuyama, C., Suwa, Y. Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O. J. Vis. Exp. (164), e59562, doi:10.3791/59562 (2020).
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