System-wide analysis of multiple biomolecules is crucial to gain functional and mechanistic insights into biological processes. Hereby, an extensive protocol is described for high throughput extraction of lipids, metabolites, proteins and starch from a single sample harvested from synchronized Chlamydomonas culture.
Mubeen, U., Giraldi, L. A., Jüppner, J., Giavalisco, P. A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii. J. Vis. Exp. (150), e59547, doi:10.3791/59547 (2019).