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神经系统疾病中的脂质组学和转录组学

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Lipidomics and Transcriptomics in Neurological Diseases

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神经系统疾病中的脂质组学和转录组学

3,159 Views

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09:58 min

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March 18, 2022

DOI:

10.3791/59423-v

DOI:

10.3791/59423-v

•

09:58 min

March 18, 2022

•

4 Views

Julia M. Post¹, Raissa Lerner¹, Claudia Schwitter¹, Beat Lutz¹, Ermelinda Lomazzo¹, Laura Bindila¹

¹Institute of Physiological Chemistry,University Medical Center of the Johannes Gutenberg University Mainz

Summary

本文提出了一种模块化方案，用于神经系统疾病小鼠模型中的组织脂质组学和转录组学以及血浆脂质组学，靶向炎症和神经元活性下的脂质，膜脂质，下游信使以及脂质功能下的mRNA编码酶/受体。概述了采样，样品处理，提取和定量程序。

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