/
/
High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
A subscription to JoVE is required to view this content. Sign in or start your free trial.
10,667 Views
•
09:42 min
•
April 30, 2019
DOI:
Summary
Automatically generated
Novel, immunostaining-compatible tissue clearing techniques like the ultimate 3D imaging of solvent-cleared organs allow the 3D visualization of rabies virus brain infection and its complex cellular environment. Thick, antibody-labeled brain tissue slices are made optically transparent to increase imaging depth and to enable 3D analysis by confocal laser scanning microscopy.
Read Article
Cite this Article
Zaeck, L., Potratz, M., Freuling, C. M., Müller, T., Finke, S. High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue. J. Vis. Exp. (146), e59402, doi:10.3791/59402 (2019).
Copy