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Verbesserte Hefe One-Hybrid Screens, Transkriptionsfaktor Bindung an menschliche DNA-Sequenzen zu identifizieren
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Genetics
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JoVE Journal Genetics
Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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Verbesserte Hefe One-Hybrid Screens, Transkriptionsfaktor Bindung an menschliche DNA-Sequenzen zu identifizieren

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7,719 Views

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11:25 min

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February 11, 2019

DOI:

10.3791/59192-v

DOI:
10.3791/59192-v

•

11:25 min
February 11, 2019

•

17 Views
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1Department of Biology,Boston University, 2Bioinformatics Program,Boston University

Summary

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Hier präsentieren wir Ihnen eine erweiterte Hefe One-Hybrid screening Protokoll um den Transkriptionsfaktoren (TFs) zu identifizieren, die an einer menschlichen DNA-Region of Interest binden können. Diese Methode nutzt eine Hochdurchsatz-Screening-Pipeline, die die Bindung von Verhören kann > 1.000 TFs in einem einzigen Experiment.

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Cite this Article

Shrestha, S., Liu, X., Santoso, C. S., Fuxman Bass, J. I. Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences. J. Vis. Exp. (144), e59192, doi:10.3791/59192 (2019).

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