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Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
JoVE Journal
Environment
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JoVE Journal Environment
Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

DOI:
10.3791/59142-v

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09:04 min

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April 18, 2019

•

,
1Oak Ridge Institute for Science and Education, National Exposure Research Laboratory,U.S. Environmental Protection Agency, 2National Exposure Research Laboratory,U.S. Environmental Protection Agency

Chapters

  • 00:04Title
  • 00:29Water Sample Collection and Extraction
  • 03:44Targeted Liquid Chromatography with Mass Spectrometry (LC-MS/MS) Quantitation
  • 05:01Non-targeted LC-MS/MS Data Collection
  • 06:50Results: Representative PFAS Chemical Species Identification
  • 08:25Conclusion

Summary

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Automatic Translation

Here, we present a protocol for the sequential targeted quantification and non-targeted analysis of fluorinated compounds in water by mass spectrometry. This methodology provides quantitative levels of known fluorochemical compounds and identifies unknown chemicals in related samples with semi-quantitative estimates of their abundance.

Tags

Keywords: Per- And Polyfluorinated ChemicalsPFASHigh-resolution Mass SpectrometryTargeted And Non-targeted ScreeningSample CollectionSample PreparationSolid-phase ExtractionQuantitationCalibrationInternal Standards

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