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气泡柱光生物反应器中绿色微藻的培养及中性脂质的测定
JoVE Journal
Environment
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JoVE Journal Environment
Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
DOI:

11:08 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:42Setup of Bubble Column Photobioreactors
  • 02:13Cultivation of Microalgae in Bubble Column Photobioreactors
  • 04:03Harvest and Freeze Drying of Microalgal Biomass
  • 05:28Lipid Extraction Using a Modified Folch Method
  • 07:07Neutral Lipid Assay Using a Microplate Method (Adapted from Higgins et al. 201422)
  • 09:01Results: Auxenochlorella protothecoides Growth Results and Chlorella sorokiniana Neutral Lipid Data
  • 10:35Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 以构建实验室规模的气泡柱光生物反应器, 并将其用于培养微藻。为测定培养生长速率和中性脂质含量提供了一种方法。

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