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Fracionamento celular de células U937, na ausência de centrifugação de alta velocidade
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Biochemistry
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JoVE Journal Biochemistry
Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Fracionamento celular de células U937, na ausência de centrifugação de alta velocidade

12,919 Views

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06:53 min

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January 18, 2019

DOI:

10.3791/59022-v

DOI:
10.3791/59022-v

•

06:53 min
January 18, 2019

•

13 Views
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1Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

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Cell FractionationU937 CellsHigh-speed CentrifugationProtocolSubcellular FractionsCytoplasmMitochondriaPlasma MembraneHypotonic BuffersDigitoninMechanical LysisDifferential CentrifugationProtein LocalizationInexpensiveStraightforward

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McCaig, W. D., Deragon, M. A., Haluska Jr,, R. J., Hodges, A. L., Patel, P. S., LaRocca, T. J. Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation. J. Vis. Exp. (143), e59022, doi:10.3791/59022 (2019).

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