Rivista
/
Biochimica
/
Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

DOI:
10.3791/59022-v

•

06:53 min

•

January 18, 2019

•

, , , , ,
1Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Capitoli

  • 00:04Titolo
  • 00:35PBS Wash
  • 01:01Cytosolic Protein Isolation
  • 02:21Cell Homogenization
  • 03:32Differential Centrifugation
  • 04:58Results: Undifferentiated U937 Cells Successfully Fractionated
  • 06:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to isolate the plasma membrane, cytoplasm and mitochondria of U937 cells without the use of high-speed centrifugation. This technique can be used to purify subcellular fractions for subsequent examination of protein localization via immunoblotting.

Tags

Cell FractionationU937 CellsHigh-speed CentrifugationProtocolSubcellular FractionsCytoplasmMitochondriaPlasma MembraneHypotonic BuffersDigitoninMechanical LysisDifferential CentrifugationProtein LocalizationInexpensiveStraightforward

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Read Article