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תא Fractionation של תאים U937, בהעדר צנטריפוגה במהירות גבוהה
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Biochemistry
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JoVE Journal Biochemistry
Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
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תא Fractionation של תאים U937, בהעדר צנטריפוגה במהירות גבוהה

12,919 Views

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06:53 min

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January 18, 2019

DOI:

10.3791/59022-v

DOI:
10.3791/59022-v

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06:53 min
January 18, 2019

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1Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Tags

Cell FractionationU937 CellsHigh-speed CentrifugationProtocolSubcellular FractionsCytoplasmMitochondriaPlasma MembraneHypotonic BuffersDigitoninMechanical LysisDifferential CentrifugationProtein LocalizationInexpensiveStraightforward

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Cite this Article

McCaig, W. D., Deragon, M. A., Haluska Jr,, R. J., Hodges, A. L., Patel, P. S., LaRocca, T. J. Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation. J. Vis. Exp. (143), e59022, doi:10.3791/59022 (2019).

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