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साइट-निर्देशित Mutagenesis इन विट्रो में और Vivo प्रयोगों उदाहरण में आरएनए बातचीत के साथ ई कोलाई
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Biochemistry
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JoVE Journal Biochemistry
Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
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साइट-निर्देशित Mutagenesis इन विट्रो में और Vivo प्रयोगों उदाहरण में आरएनए बातचीत के साथ ई कोलाई

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07:04 min

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February 05, 2019

DOI:

10.3791/58996-v

DOI:
10.3791/58996-v

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07:04 min
February 05, 2019

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7 Views
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1Department of Biochemistry and Molecular Biology,University of Southern Denmark

Tags

Site-directed MutagenesisRNA InteractionsEscherichia ColiPCRDNA TemplatePrimer PairsAgarose Gel ElectrophoresisDNA PurificationSpectrophotometry2-step PCR3-step PCRRNA-RNA InteractionsRNA-protein Interactions

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Andreassen, P. R., Pettersen, J. S., Jørgensen, M. Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli. J. Vis. Exp. (144), e58996, doi:10.3791/58996 (2019).

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