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キイロタマホコリカビの選択に基づいて細胞と細菌の成長の遺伝子工学

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

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キイロタマホコリカビの選択に基づいて細胞と細菌の成長の遺伝子工学

12,404 Views

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06:08 min

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January 25, 2019

DOI:

10.3791/58981-v

DOI:

10.3791/58981-v

•

06:08 min

January 25, 2019

•

11 Views

Peggy Paschke¹, David A. Knecht², Thomas D. Williams¹, Peter A. Thomason³, Robert H. Insall³, Jonathan R. Chubb^4,5, Robert R. Kay¹, Douwe M. Veltman¹

¹MRC Laboratory of Molecular Biology, ²Department of Molecular and Cell Biology,University of Connecticut, ³Cancer Research UK Beatson Institute Glasgow, ⁴MRC Laboratory for Molecular Cell Biology,University College London, ⁵Department of Cell and Developmental Biology,University College London

Summary

キイロタマホコリカビは細胞遊走、エンドサイトーシス、開発など複雑な細胞プロセスを勉強する人気モデル生物です。生物のユーティリティは、遺伝子操作の可能性に依存しています。液体培地における培養細胞の既存の制限を克服するキイロタマホコリカビの細胞を使っての方法を紹介します。

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