Journal
/
Immunology and Infection
/
Un saggio di citotossicità a base di piastre per la valutazione della funzione citolitica della cellula killer naturale del ratto placentare
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function
Please note that all translations are automatically generated. Click here for the English version.

Un saggio di citotossicità a base di piastre per la valutazione della funzione citolitica della cellula killer naturale del ratto placentare

5,643 Views

•

10:44 min

•

June 02, 2019

DOI:

10.3791/58961-v

DOI:
10.3791/58961-v

•

10:44 min
June 02, 2019

•

2 Views
, , ,
1Department of Emergency Medicine,University of Mississippi Medical Center, 2Department of Experimental Therapeutics and Pharmacology,University of Mississippi Medical Center

Tags

– Plate-based Cytotoxicity Assay- Rat Placental Natural Killer Cells- Cytolytic Function- Cytotoxic Activity- Inexpensive Method- Specialized Equipment- Hazardous Materials- CD8+ Cytolytic Cells- Target-effector Cell Ratio Optimization- Mononuclear Cell Isolation- Placenta Homogenization- Density Gradient Centrifugation- Biotin-labeled CD3 Antibody- Magnetic Bead Separation

Read Article

Cite this Article

Baik, C. H., Geer, M., Travis, O. K., Cornelius, D. C. A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function. J. Vis. Exp. (148), e58961, doi:10.3791/58961 (2019).

Download .ris file

Copy

Share Video

.

Copy