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A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

5,654 Views

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10:44 min

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June 02, 2019

DOI:

10.3791/58961-v

DOI:
10.3791/58961-v

•

10:44 min
June 02, 2019

•

1 Views
, , ,
1Department of Emergency Medicine,University of Mississippi Medical Center, 2Department of Experimental Therapeutics and Pharmacology,University of Mississippi Medical Center

Summary

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Here, we provide a detailed methodology to isolate and assess cytotoxic function of natural killer cells from placentas by a colorimetric plate assay. The reduced uterine perfusion pressure rat model of placental ischemia was chosen to demonstrate the antibody-mediated isolation and assessment of the cytotoxic function of natural killer cells.

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Cite this Article

Baik, C. H., Geer, M., Travis, O. K., Cornelius, D. C. A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function. J. Vis. Exp. (148), e58961, doi:10.3791/58961 (2019).

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