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En plade-baseret cytotoksicitet analyse til vurdering af rotte Placental naturlig Killer Cell cytolytisk funktion
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

En plade-baseret cytotoksicitet analyse til vurdering af rotte Placental naturlig Killer Cell cytolytisk funktion

5,643 Views

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10:44 min

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June 02, 2019

DOI:

10.3791/58961-v

DOI:
10.3791/58961-v

•

10:44 min
June 02, 2019

•

2 Views
, , ,
1Department of Emergency Medicine,University of Mississippi Medical Center, 2Department of Experimental Therapeutics and Pharmacology,University of Mississippi Medical Center

Tags

– Plate-based Cytotoxicity Assay- Rat Placental Natural Killer Cells- Cytolytic Function- Cytotoxic Activity- Inexpensive Method- Specialized Equipment- Hazardous Materials- CD8+ Cytolytic Cells- Target-effector Cell Ratio Optimization- Mononuclear Cell Isolation- Placenta Homogenization- Density Gradient Centrifugation- Biotin-labeled CD3 Antibody- Magnetic Bead Separation

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Cite this Article

Baik, C. H., Geer, M., Travis, O. K., Cornelius, D. C. A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function. J. Vis. Exp. (148), e58961, doi:10.3791/58961 (2019).

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