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Detecção de tempo real em Vitro Tumor celular apoptose induzida por CD8+ T células para estudar as funções imunológicas supressora de Tumor infiltrando células mieloides
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Detecção de tempo real em Vitro Tumor celular apoptose induzida por CD8+ T células para estudar as funções imunológicas supressora de Tumor infiltrando células mieloides

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21,584 Views

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09:57 min

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January 29, 2019

DOI:

10.3791/58841-v

DOI:
10.3791/58841-v

•

09:57 min
January 29, 2019

•

7 Views
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1Royal (Dick) School of Veterinary Studies and Roslin Institute,University of Edinburgh, 2MRC Centre for Reproductive Health,University of Edinburgh, 3Edinburgh Phenotypic Assay Centre,University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine,University of Edinburgh

Summary

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Descrevemos aqui um protocolo para investigar a citotoxidade de CD8 pré-activated+ T células contra as células cancerosas através da detecção de apoptotic câncer células através do tempo real microscopia. Este protocolo pode investigar os mecanismos subjacentes mieloide induzida por células T cell supressão e avaliar compostos vistos reabastecer T células através do bloqueio de células mieloides supressivas do sistema imunológico.

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Kitamura, T., Doughty-Shenton, D., Pollard, J. W., Carragher, N. O. Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells. J. Vis. Exp. (143), e58841, doi:10.3791/58841 (2019).

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