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Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

English

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6,162 Views

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10:09 min

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November 06, 2018

DOI:

10.3791/58603-v

DOI:
10.3791/58603-v

•

10:09 min
November 06, 2018

•

1 Views
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1Department of Ocean Science,Hong Kong University of Science and Technology, 2Division of Life Science,Hong Kong University of Science and Technology, 3HKUST Shenzhen Research Institute

Summary

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Here we describe a photobleaching method to reduce the autofluorescence of cyanobacteria. After photobleaching, stochastic optical reconstruction microscopy is used to obtain three-dimensional super-resolution images of the cyanobacterial FtsZ ring.

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Zhan, Y., Liu, Y., Zeng, Q. Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus. J. Vis. Exp. (141), e58603, doi:10.3791/58603 (2018).

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