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Cuantificación automatizada basada en imágenes de trampas extracelulares de neutrófilos mediante NETQUANT
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Immunology and Infection
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JoVE Journal Immunology and Infection
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
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Cuantificación automatizada basada en imágenes de trampas extracelulares de neutrófilos mediante NETQUANT

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6,544 Views

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07:33 min

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November 27, 2019

DOI:

10.3791/58528-v

DOI:
10.3791/58528-v

•

07:33 min
November 27, 2019

•

3 Views
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1Department of Clinical Sciences, Division of Infection Medicine,Lund University

Summary

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Aquí, presentamos un protocolo para generar trampas extracelulares de neutrófilos (NET) y operar NETQUANT, una opción de software totalmente automática para la cuantificación de NETs en imágenes de inmunofluorescencia.

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NETQUANTAutomated Image AnalysisNeutrophil Extracellular Traps (NETs)Immunofluorescence ImagingImage SegmentationAdaptive SegmentationDNA ChannelNET ChannelSample TypesBatch ProcessingImage MetadataZenodoNordonfelt Lab

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Cite this Article

Mohanty, T., Nordenfelt, P. Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT. J. Vis. Exp. (153), e58528, doi:10.3791/58528 (2019).

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