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Identifikation der homologen Rekombinationsereignisse in embryonalen Stammzellen der Maus mit Southern Blotting und Polymerase-Kettenreaktion
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Genetics
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JoVE Journal Genetics
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
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Identifikation der homologen Rekombinationsereignisse in embryonalen Stammzellen der Maus mit Southern Blotting und Polymerase-Kettenreaktion

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9,020 Views

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08:01 min

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November 20, 2018

DOI:

10.3791/58467-v

DOI:
10.3791/58467-v

•

08:01 min
November 20, 2018

•

2 Views
, , , , , , , , ,
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine,Hunan Agricultural University (HUNAU), 2Department of Pathology,Georgetown University Medical School, 3College of Food Science and Technology,Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC),National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core,National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)

Summary

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Hier präsentieren wir Ihnen ein detailliertes Protokoll zur Identifizierung von homologen Rekombinationsereignisse in embryonalen Stammzellen der Maus mit Southern blotting und/oder PCR. Diese Methode ist durch die Generierung von Nonmuscle Myosin II genetische Ersatz Mausmodellen mit traditionellen embryonalen Stammzell-basierte homologe Rekombination-vermittelten targeting-Technologie veranschaulicht.

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Homologous RecombinationMouse Embryonic Stem CellsSouthern BlottingPolymerase Chain ReactionMolecular BiologyCellular BiologyGenetically Modified CellsGDNA DigestionAgarose Gel ElectrophoresisDNA TransferDNA LabelingDNA Hybridization

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Cite this Article

Zhou, D., Tan, L., Li, J., Liu, T., Hu, Y., Li, Y., Kawamoto, S., Liu, C., Guo, S., Wang, A. Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction. J. Vis. Exp. (141), e58467, doi:10.3791/58467 (2018).

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