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第三星果蝇中枢神经系统活动的电生理记录
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Neuroscience
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JoVE Journal Neuroscience
Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster
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第三星果蝇中枢神经系统活动的电生理记录

DOI:
10.3791/58375-v

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06:45 min

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November 21, 2018

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, , ,
1Department of Entomology,Louisiana State University AgCenter, 2Department of Entomology,Virginia Tech, 3Department of Entomology and Nematology, Emerging Pathogens Institute,University of Florida

Chapters

  • 00:04Title
  • 00:35Preparation of Software for Dissection and Recording
  • 02:05Dissect and Prepare the Larval Drosophila CNS
  • 03:35Extracellular Recording of Drosophila CNS
  • 05:22Results: Neurophysiological Recordings from the CNS of Third-Instar Larvae of D. melanogaster
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种方法来记录果蝇中枢神经系统的电活动下降, 以实现对药理剂、神经蛋白基因突变的经济高效和方便的检测,和尚未探索的生理途径的作用。

Tags

Electrophysiological RecordingCentral Nervous SystemDrosophila MelanogasterInsect ToxicologyInsect PhysiologyElectrogenesisInsecticide Modes Of ActionNervous SystemAcquisition/analysis SoftwareRaw SignalAmplifierEvent CountingThresholdRate PlotHertzDissectionThird-instar LarvaeSalineForcepsCentral Nervous System Extraction

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