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Kweken en manipulatie van O9-1 neurale kamcellen
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Genetics
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JoVE Journal Genetics
Culturing and Manipulation of O9-1 Neural Crest Cells
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Kweken en manipulatie van O9-1 neurale kamcellen

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9,436 Views

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08:32 min

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October 09, 2018

DOI:

10.3791/58346-v

DOI:
10.3791/58346-v

•

08:32 min
October 09, 2018

•

5 Views
, , ,
1Molecular Physiology and Biophysics,Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology,University of Southern California

Summary

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O9-1 is een cellijn van multipotente muis crest van de neurale. Hier beschrijven we gedetailleerde stapsgewijze protocollen voor kweken O9-1 cellen, O9-1 cellen in specifieke celtypes te differentiëren en genetisch manipuleren O9-1 cellen met behulp van siRNA-gemedieerde knockdown of bewerken van CRISPR-Cas9-genoom.

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Cite this Article

Nguyen, B. H., Ishii, M., Maxson, R. E., Wang, J. Culturing and Manipulation of O9-1 Neural Crest Cells. J. Vis. Exp. (140), e58346, doi:10.3791/58346 (2018).

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