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散逸の Microgravimetry 結合タンパク質アネキシン A2 への水晶振動子を用いた固相担脂質膜リン脂質の結合動態を研究するには
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
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散逸の Microgravimetry 結合タンパク質アネキシン A2 への水晶振動子を用いた固相担脂質膜リン脂質の結合動態を研究するには

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6,745 Views

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07:11 min

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November 01, 2018

DOI:

10.3791/58224-v

DOI:
10.3791/58224-v

•

07:11 min
November 01, 2018

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6 Views
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1Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation,University of Münster, 2Institute of Biochemistry,University of Münster, 3Cluster of Excellence ‘Cells in Motion’,University of Münster

Summary

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ここでは、結合親和性と固定化固相担膜 (SLB) と A2 を同時に測定することによりアネキシン ラベル無料リン脂質結合蛋白質の相互作用のモードを決定するために用いることができる実験的プロトコルを提案する、質量吸収とアネキシン A2 蛋白質の粘弾性特性。

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Matos, A. L. L., Grill, D., Kudruk, S., Heitzig, N., Galla, H., Gerke, V., Rescher, U. Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator. J. Vis. Exp. (141), e58224, doi:10.3791/58224 (2018).

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