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Quantitative Zellbiologie der Neurodegeneration in Drosophila durch unvoreingenommene Analyse der Gewebekulturen Tagged Proteine mit ImageJ
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1Department of Molecular and Cellular Pharmacology,University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Yantai University
Chapters
- 00:04Title
- 00:50Brain Dissection and Immunofluorescence Staining
- 02:23Image Acquisition and Region of Interest (ROI) Selection
- 04:19Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification
- 06:30Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration
- 08:20Conclusion
Wir haben einen einfachen und anpassungsfähigen Workflow zur Fluoreszenz-Imaging-basierte zellbiologischen Studien Proteinaggregation und selbstverdauende Fluss in das zentrale Nervensystem der Drosophila -Modelle von quantitativen Daten entlocken entwickelt. Neurodegeneration.
Tags
Keywords:
Quantitative Cell BiologyNeurodegenerationDrosophilaFluorescently Tagged ProteinsImageJImage Analysis WorkflowProteinaceous PunctaMembrane-bound CompartmentsNeurodegenerative DiseasesLamina DissectionRetina RemovalBrain DissectionImmunostainingAntibody IncubationMountingMicroscopyFluorescence Imaging
