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The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression
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Genetics
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JoVE Journal Genetics
The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

English

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7,355 Views

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06:50 min

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July 24, 2018

DOI:

10.3791/58022-v

DOI:
10.3791/58022-v

•

06:50 min
July 24, 2018

•

9 Views
1Department of Biology,University of Hartford

Summary

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This protocol describes a technique using mouse splenocytes to discover pathogen-associated molecular patterns that alter molecular clock gene expression.

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Mouse SplenocytesPathogen-associated Molecular PatternClock Gene ExpressionImmunologyChronobiologyPAMPCell CultureCell HomogenizationCell CountingCell StimulationRNA Extraction

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Cite this Article

Silver, A. C. The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression. J. Vis. Exp. (137), e58022, doi:10.3791/58022 (2018).

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