/

/

调频染料循环在突触: 高钾偏极化, 电和 Channelrhodopsin 刺激的比较

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation

Please note that all translations are automatically generated. Click here for the English version.

调频染料循环在突触: 高钾偏极化, 电和 Channelrhodopsin 刺激的比较

DOI:

10.3791/57765-v

•

08:31 min

•

May 24, 2018

•

Danielle L. Kopke, Kendal Broadie

¹Department of Biological Sciences,Vanderbilt University, ²Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development,Vanderbilt University and Medical Center

Chapters

00:04Title
00:42Larval Glue Dissection
02:36Electrical Stimulation: FM Dye Loading and Unloading
04:08Channelrhodopsin Stimulation: FM Dye Loading and Unloading
05:21Fluorescence Quantification
06:12Results: Synaptic Ultrastructure with FM Dye Photoconversion to Mark Vesicles
07:22Conclusion

Summary

Automatic Translation

突触囊泡 (SV) 循环是神经元突触中细胞间通讯的核心机制。调频染料的摄取和释放是定量测定 SV 内胞吐作用的主要手段。在这里, 我们比较所有的刺激方法, 以驱动 FM1-43 循环在果蝇神经肌肉连接 (NMJ) 模型突触。

Tags

FM Dye Synaptic Vesicle Cycling Neuromuscular Junction Drosophila Larvae High Potassium Depolarization Electrical Stimulation Optogenetics Anti-HRP Antibody Elastomer-coated Coverslip Dissection Gluing Motor Nerve Calcium-free Saline Suction Pipette Microelectrode Puller Micro Forge Micromanipulator

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

结合经颅磁刺激和功能磁共振成像检查默认模式网络

依赖国家对TMS的：一看动机幻视行为

与耳蜗内毛细胞传入树突：多泡发布监测功能区突触的突触后录音

调查外毛细胞运动与外部的交流电场刺激和高速图像分析相结合

监测劈开caspase - 3活性和钾诱导膜去极化使用活细胞成像和Cleaveable荧光染料基板的永生的少突胶质细胞凋亡

神经肌肉功能的应用经皮神经电刺激评估

时间依赖性增加对微电极阵列刺激神经元细胞培养物的网络响应

警惕啮齿动物的经颅电脑刺激

大鼠慢性经颅电刺激及 Intracortical 记录

侧前额叶皮质联合经颅磁刺激与脑电图的研究

Read Article