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Journal
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Biochemistry
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原位
近接结扎法检测蛋白异构体的 Heterodimerization
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Detection of Heterodimerization of Protein Isoforms Using an
in Situ
Proximity Ligation Assay
Please note that all translations are automatically generated.
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原位
近接结扎法检测蛋白异构体的 Heterodimerization
DOI:
10.3791/57755-v
•
09:18 min
•
October 20, 2018
•
Sofiia Karchugina
,
Jonathan Chernoff
1
Fox Chase Cancer Center
Chapters
00:02
Title
02:19
Fixation, Permeabilization and Blocking of Cells
03:29
Primary Antibodies
04:15
PLA Probes
04:34
Ligation
05:27
Amplification
06:19
Preparation for Imaging
07:31
Results
08:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们展示了如何使用接近结扎法 (PLA) 在高灵敏度的固定细胞中可视化 MST1/MST2 heterodimerization。
Tags
Protein Isoforms
In Situ Proximity Ligation Assay
PLA
Protein Interactions
Heterodimers
MST1
MST2
Fluorescence Signal
Microscope Imaging
Cell Culture
Cell Fixation
Cell Permeabilization
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