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Deteção de pequena GTPase Prenilação e GTP ligação usando o fracionamento de membrana e ensaio de imunoabsorção ligada de GTPase
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13:51 min
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November 11, 2018
DOI:
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine,University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba,University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences,Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine,Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec,Medical University of Silesia, 7Centre de biophysique moléculaire – UPR 4301,Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children’s Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences,University of Manitoba, 10Health Policy Research Center, Institute of Health,Shiraz University of Medical Sciences
Summary
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Aqui descrevemos um protocolo para investigar a Prenilação e guanosina-5'-trifosfato (GTP)-carregamento de GTPase. Esse protocolo consiste em dois métodos detalhados, nomeadamente o fracionamento de membrana e uma GTPase-lig da imunoabsorção do ensaio. O protocolo pode ser utilizado para medir a Prenilação e GTP carregamento de diferentes outras GTPases pequenas.
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Cite this Article
Alizadeh, J., Shojaei, S., da Silva Rosa, S., Rezaei Moghadam, A., Zeki, A. A., Hashemi, M., Los, M. J., Gordon, J. W., Ghavami, S. Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay. J. Vis. Exp. (141), e57646, doi:10.3791/57646 (2018).
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