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Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
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Cancer Research
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JoVE Journal Cancer Research
Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

7,763 Views

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11:11 min

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April 25, 2018

DOI:

10.3791/57516-v

DOI:
10.3791/57516-v

•

11:11 min
April 25, 2018

•

1 Views
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1Laboratory of Molecular Oncology, Division of Thoracic Surgery,University Hospital Zurich

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DesthiobiotinStreptavidinAffinity PurificationRNA-interacting ProteinsMesothelioma CellsCytosolic ProteinRNA BindingReporter AssayACC-MESO-4 CellsTrypsin-EDTARPMI-1640 MediumPBSCytoplasmic ExtractionProteinase InhibitorNuclear Extraction

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Kresoja-Rakic, J., Felley-Bosco, E. Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells. J. Vis. Exp. (134), e57516, doi:10.3791/57516 (2018).

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