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巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites
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巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法

DOI:
10.3791/57486-v

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10:01 min

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March 16, 2018

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, , , ,
1Department of Cell Biology and Molecular Genetics,University of Maryland

Chapters

  • 00:04Title
  • 01:08Bone Marrow-derived Macrophage (BMM) Isolation and Differentiation
  • 04:05BMM Coverslip Plating
  • 05:22L. amazonensis Infection and Parasite Quantification
  • 07:21Results: Representative BMM Infection Analyses
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

所有致病性的利什曼原虫物种在其脊椎动物宿主的巨噬细胞内栖息和复制。在这里, 我们提出了一个协议, 以感染小鼠骨髓源性巨噬细胞的文化与利什曼原虫, 然后精确定量的细胞内生长动力学。该方法对于研究影响寄主病原体相互作用和利什曼原虫毒力的个体因素非常有用。

Tags

MacrophageLeishmaniaVirulenceIntracellular GrowthBone Marrow-derivedIn VitroMurineParasite QuantificationTherapyLeishmaniasisMutated StrainsTherapeutic CompoundsIsolationDissection

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