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Digestione delle proteine, ultrafiltrazione e cromatografia di esclusione di formato per ottimizzare l'isolamento degli esosomi da siero e Plasma sanguigno umano
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Biochemistry
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JoVE Journal Biochemistry
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
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Digestione delle proteine, ultrafiltrazione e cromatografia di esclusione di formato per ottimizzare l'isolamento degli esosomi da siero e Plasma sanguigno umano

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09:22 min

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April 13, 2018

DOI:

10.3791/57467-v

DOI:
10.3791/57467-v

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09:22 min
April 13, 2018

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6 Views
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1Department of Microbiology, Immunology and Pathology,Colorado State University, 2Department of Biological Sciences, Eck Institute for Global Health,University of Notre Dame

Tags

Protein DigestionUltrafiltrationSize Exclusion ChromatographyExosome IsolationBlood PlasmaSerumProteomicsBiomarkersExtracellular VesiclesProteinase K

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Diaz, G., Bridges, C., Lucas, M., Cheng, Y., Schorey, J. S., Dobos, K. M., Kruh-Garcia, N. A. Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum. J. Vis. Exp. (134), e57467, doi:10.3791/57467 (2018).

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