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Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
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Biochimie
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Journal JoVE Biochimie
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

16,041 Views

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09:22 min

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April 13, 2018

DOI:

10.3791/57467-v

DOI:
10.3791/57467-v

•

09:22 min
April 13, 2018

•

6 Views
, , , , , ,
1Department of Microbiology, Immunology and Pathology,Colorado State University, 2Department of Biological Sciences, Eck Institute for Global Health,University of Notre Dame

Tags

Protein DigestionUltrafiltrationSize Exclusion ChromatographyExosome IsolationBlood PlasmaSerumProteomicsBiomarkersExtracellular VesiclesProteinase K

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Diaz, G., Bridges, C., Lucas, M., Cheng, Y., Schorey, J. S., Dobos, K. M., Kruh-Garcia, N. A. Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum. J. Vis. Exp. (134), e57467, doi:10.3791/57467 (2018).

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