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Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
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Environment
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JoVE Journal Environment
Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

English

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12,583 Views

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05:46 min

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August 01, 2018

DOI:

10.3791/57434-v

DOI:
10.3791/57434-v

•

05:46 min
August 01, 2018

•

3 Views
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1Niederrhein University of Applied Sciences, 2University Duisburg-Essen

Summary

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We present a protocol for non-targeted analysis using time of flight mass spectrometry as a perfect tool to identify pharmaceuticals in waters. We demonstrate the application of UV irradiation for their elimination. Analysis involving irradiation, compound isolation, identification and kinetic modelling of the degradation profiles is illustrated.

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HPLC-ESI-Q-TOF-MSPharmaceuticalsAquatic EnvironmentErythromycinPhoto-induced DegradationSolid Phase ExtractionFiltrationRotary EvaporationNon-targeted AnalysisUV IrradiationPhotoreactorPH AdjustmentTime-course Sampling

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Voigt, M., Savelsberg, C., Jaeger, M. Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation. J. Vis. Exp. (138), e57434, doi:10.3791/57434 (2018).

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