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Isolamento di cellule endoteliali umane da Colon normale e Carcinoma colorettale - un protocollo migliorato
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April 04, 2018
DOI:
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg
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Cellule endoteliali tumorali sono fattori determinanti importanti del microambiente tumorale e il decorso della malattia. Qui, un protocollo per l'isolamento di cellule endoteliali pure e praticabile da carcinoma colorettale umano e colon normale a essere impiegata nella ricerca di droga test e la patogenesi è descritto.
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Naschberger, E., Regensburger, D., Tenkerian, C., Langheinrich, M., Engel, F. B., Geppert, C., Hartmann, A., Grützmann, R., Schellerer, V. S., Stürzl, M. Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol. J. Vis. Exp. (134), e57400, doi:10.3791/57400 (2018).
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