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February 02, 2018
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Here, we present a protocol for studying plant growth behavior and especially phenotypes in a reproducible manner. We show how to provide variable and at the same time stable light conditions. Proper analyses depend on sufficient sample numbers and valid statistical evaluations.
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Arabidopsis ThalianaPlant GrowthLight ConditionsGenotypesVisual Phenotype AnalysisMolecular Phenotype AnalysisLight AcclimationReproducible ResultsQuantifiable ResultsSample SizeVernalizationClimatic ChamberRelative Air HumidityTemperatureLight IntensitySpectral OutputPlant Growth DataPulse-modulated FluorometerFluorescence ImagingArea Of Interest (AOI)Maximum Photosystem II Quantum Yield (Fv/Fm)
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Bölter, B., Seiler, F., Soll, J. Analysis of Arabidopsis thaliana Growth Behavior in Different Light Qualities. J. Vis. Exp. (132), e57152, doi:10.3791/57152 (2018).
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