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Kinetische analyse van vasculogenese kwantificeert dynamiek van Vasculogenese en angiogenese In Vitro
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January 31, 2018
DOI:
1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine
Summary
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Hier presenteren we een protocol voor time-lapse beeldvorming en analyse van het vasculogenese in vitro gebruik van fase contrast microscopie in combinatie met de open source-software, kinetische analyse van vasculogenese. Dit protocol kan worden toegepast om het kwantitatief het beoordelen van het potentieel van de vasculogenic van vele celtypes of experimentele omstandigheden dat model vasculaire ziekte.
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Varberg, K. M., Winfree, S., Dunn, K. W., Haneline, L. S. Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro. J. Vis. Exp. (131), e57044, doi:10.3791/57044 (2018).
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