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A Method for Targeted 16S Sequencing of Human Milk Samples
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Method for Targeted 16S Sequencing of Human Milk Samples

A Method for Targeted 16S Sequencing of Human Milk Samples

9,674 Views

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09:09 min

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March 23, 2018

DOI:

10.3791/56974-v

DOI:
10.3791/56974-v

•

09:09 min
March 23, 2018

•

7 Views
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1Department of Pediatrics,University of California at Los Angeles

Tags

16S SequencingHuman MilkLow Biomass SamplesSemi-automated ProtocolMicrobiomeCell PelletLysis BufferBead BeatingDNA ExtractionPCR Amplification

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Tobin, N. H., Woodward, C., Zabih, S., Lee, D. J., Li, F., Aldrovandi, G. M. A Method for Targeted 16S Sequencing of Human Milk Samples. J. Vis. Exp. (133), e56974, doi:10.3791/56974 (2018).

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