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结合 x 射线晶体学与小角度 x 射线散射到模型的非结构化区域的 Nsa1 从s 酿酒酵母
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Biochemistry
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JoVE Journal Biochemistry
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
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结合 x 射线晶体学与小角度 x 射线散射到模型的非结构化区域的 Nsa1 从s 酿酒酵母

DOI:
10.3791/56953-v

•

09:15 min

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January 10, 2018

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, ,
1Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Chapters

  • 00:05Title
  • 00:47Nsa1 (Nop7 Associated 1) Protein Purification
  • 03:43Proteolytic Screening
  • 05:14Small-Angle X-Ray Scattering (SAXS) Data Processing, Comparison of Concentration Series, and Ensemble Modeling
  • 07:31Results: Modeling the Disordered C-Terminus Region of Nsa1
  • 08:37Conclusion

Summary

Automatic Translation

Automatic Translation

该方法描述了用 x 射线晶体学和小角 x 射线散射 (SAXS) 进行结构测定的重组 Nsa1 的克隆、表达和纯化, 适用于其他蛋白质的混合结构分析包含有序和无序的域。

Tags

X-ray CrystallographySmall Angle X-ray Scattering (SAXS)Structural AnalysisProtein StructureRecombinant ProteinUnstructured RegionsNsa1S. CerevisiaeHybrid Structural AnalysisProtein FunctionProtein DynamicsProtein AggregationPurificationAffinity ChromatographyGel FiltrationSDS-PAGETEV ProteaseCentrifugal Filtration

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