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Bir iyon kanal protein biyo-katman Interferometry tahlil tarafından küçük molekülleri ile etkileşimi Kinetik yakalama
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Biochemistry
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JoVE Journal Biochemistry
Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
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Bir iyon kanal protein biyo-katman Interferometry tahlil tarafından küçük molekülleri ile etkileşimi Kinetik yakalama

Türkçe

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8,031 Views

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10:41 min

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March 07, 2018

DOI:

10.3791/56846-v

DOI:
10.3791/56846-v

•

10:41 min
March 07, 2018

•

3 Views
, , ,
1Department of General Surgery, Tongren Hospital,Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine,Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital,Shanghai Jiao Tong University School of Medicine

Summary

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İletişim kuralı küçük molekül lipid ligand phosphatidylinositol 4, 5-bisphosphate (PIP2) ile arıtılmış hEAG1 iyon kanal protein etkileşimleri açıklar. Ölçüm BLI roman küçük molekül iyon kanal ligand tarama için potansiyel bir yöntem olabilir gösterir.

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Cite this Article

Han, B., Zhang, M., Sun, P., Hou, S. Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay. J. Vis. Exp. (133), e56846, doi:10.3791/56846 (2018).

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