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Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms
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Neuroscience
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JoVE Journal Neuroscience
Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms

Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms

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6,652 Views

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06:40 min

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January 25, 2018

DOI:

10.3791/56795-v

DOI:
10.3791/56795-v

•

06:40 min
January 25, 2018

•

2 Views
, , , , , ,
1Laboratory of Molecular Neurobiology, Institute of Cellular and Organismic Biology,Academia Sinica

Summary

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We have successfully generated two substrate-specific γ-secretase assays. Both cell-based assays presented here are designed to quantify γ-secretase enzymatic activities via the output of firefly luciferase reporters.

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Wang, B., Wu, P., Chen, Y., Chang, Y., Bhore, N., Wu, P., Liao, Y. Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms. J. Vis. Exp. (131), e56795, doi:10.3791/56795 (2018).

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