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饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡
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Immunology and Infection
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JoVE Journal Immunology and Infection
Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
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饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡

English

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9,089 Views

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08:26 min

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October 31, 2017

DOI:

10.3791/56535-v

DOI:
10.3791/56535-v

•

08:26 min
October 31, 2017

•

2 Views
, , ,
1Department of Microbiology and Immunology,University of Louisville

Summary

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本文阐述了一种从骨髓细胞中提取小鼠原发巨噬蛋白的方法, 并提出了制备 BSA-脂肪酸共轭物的简便方法。然后我们证明饱和脂肪酸能诱导巨噬细胞死亡, 这种细胞死亡与神经酰胺水平的细胞积累呈正相关。

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Cite this Article

Zhang, Y., Hao, J., Sun, Y., Li, B. Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death. J. Vis. Exp. (128), e56535, doi:10.3791/56535 (2017).

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