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Immunologie und Infektion
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Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

DOI:
10.3791/56535-v

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08:26 min

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October 31, 2017

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, , ,
1Department of Microbiology and Immunology,University of Louisville

Kapitel

  • 00:05Titel
  • 00:59Preparation of Mouse Bone Marrow Derived Macrophages (BMDMs) and BSA-fatty Acid Conjugate
  • 04:31Saturated Fatty Acids Induce Cell Death of Mouse Bone Marrow Derived Macrophages
  • 06:04Intracellular Ceramide Staining
  • 07:03Results: Fatty Acid-induced Macrophage Cell Death and Ceramide Production
  • 07:47Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We illustrate a straight-forward method to derive murine primary macrophages from bone marrow cells and a simple method to prepare BSA-fatty acid conjugates. Then we demonstrate that saturated fatty acids can induce macrophage cell death, and such cell death is positively associated with cellular accumulation of ceramide levels.

Tags

Saturated Fatty AcidsMacrophagesCeramidesCell DeathMetabolic InflammationBSA Fatty Acid ConjugatesBone Marrow CellsRed Blood Cell LysisRPMI 1640 MediumMacrophage DifferentiationM-CSF

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